LIST OF TECHNOLOGIES GENERATED FOR POSSIBLE PROMOTION OR COMMERCIALIZATION

Title of Technology

Brief Description

Project/ Program Source

Agency

Researcher/s

Classification

 

Air-Fed Wood-Fueled Stove

One of a kind stove that eliminates the problems encountered in cooking food using firewood such as uneven burning of firewood, too much smoke and continuous burning. The air-fed wood-fueled stove is fitted with a wooden, double-acting pump that injects abundant supply of air to support fuel combustion, thus eliminating too much smoke. It also maximizes the utilization of the heat content of the wood fuel. Thus, this stove is environment friendly and efficient and economical.

 

 

CSC

 

Engr. Jose T. Tapel

 

Developed;

For commercialization

Improvised Air compressor

 

Functional, inexpensive, efficient and safe air compressor designed to operate a spray gun for car painting jobs and related projects in the shop. The device is composed of a refrigerator motor with an oil and water separator, air hose to be connected to the spray gun while the body is cart-like for easy transporting. The device operates on a principle of compression. The rotary motion of the motor reciprocates the piston that travel in the cylinder to produce air.

 

CSC

Prof. Jesus Tomas M. Gianan

Developed;

For commercialization

Improvised Microbiological Incubator

The improvised incubator is a major equipment in teaching Microbiology topics on culture of microorganisms. With the use of this device, it allows the students greater opportunity to work maximally in manipulating laboratory culture materials and equipment. The improvised microbiological incubator is economical without sacrificing precise temperature control. It is stackable for maximum space utilization. Four unadjustable feet ensure proper leveling.

 

CSC

Dr. Asuncion V. San Juan

Prof. Estrella T. Tribiana

 

Multi-purpose Natural Straw Stripper

 

A device used in stripping caragumay or other natural straws used in making cottage or handicraft products. It is easy to operate and safe to use. The device, powered by ¼ hp motor, has a stripping efficiency rating of 97% which can produce 20 atrips in one setting at a maximum time of 30 seconds- a great advantage over the use of the traditional method which takes 25 minutes to produce the same number of strips. The use of recyclable materials makes the device affordable and easy to replicate; its adjustable parts allow stripping variety of natural straws, hence, it becomes multi-purpose.

 

CSC

Engr. Pio G. Panti

Engr. Jhomann Socito

Prof. Ananias Fernandez

Dr. Asuncion V. Asetre

Prof. Sonia R. Vargas

Developed;

For commercialization

Pili Nut Cutter

Mechanically operated pili nut cutter. Efficiency of traditional method of cutting pili shells increased by 10 times. Safety of cutting ensured.

 

CSC

Dr. Maria Tugano

Prof. Rhodora Cabrera

Prof. Jesus Tomas Gianan

Mark Molina

Edmund Cornelius Manalgñit

Mark Anthony Tan

Paul John Francia

Raymund Tuquero

 

Triodical Triangles as Teaching Device in Trigonometry

 

It is a teaching device that can be considered as the summary of trigonometry. It serves as a concrete example of the application of radical expressions. It is handy, affordable, and easy to reproduce.

 

CSC

Prof. Serelino T. Tasarra

Developed;

For commercialization

Coco Jam (Santan)

 

A kind of dessert made from a mixture of coco milk and panocha standardized to establish the best proportion and shelf life of the product

 

CSC

Prof. Socorro D. Masagca

Developed;

For commercialization

Value Added Products from Eucheuma

Developed products using eucheuma namely chutney, pickles, and quickles. From among the three products, chutney was the most acceptable

 

CSC

Prof. Sonia R. Vargas

Ms. Ma. Theresa I. Lacson

 

Mungbean Noodles

Use of 100% mungbean flour for production of best quality noodle. The formulation is found to have comparable qualities with the 100% all-purpose flour available in the market in terms of color, glossiness and doneness.

 

CSC

Prof. Elena G. Tasarra

Ms. Mary Ann M. Tejada

 

Pili Pulp Catsup

100% pili pulp was formulated into Pili Pulp Catsup. The color consistency, texture, flavor were standardized. Acceptability of the product was evaluated. Shelf life of 3-4 months was recorded.

 

CSC

Prof. Elena G. Tasarra

Dr. Minerva I. Morales

 

Eucheuma Farming System

To produce a yield of nine time its initial biomass, Echeuma striatum should be cultured on a farm site with mean salinity of more that 35.5ppm, a mean depth of 5.6 meters or more, a mean surface temperature of not more that 28.5 degree Celsius and a mean water velocity of not less that 0.46 m/s during the dry season.

 

CSC

Prof. Sonia r. Vargas

 

Use of Photoperiod in Mudcrab Fattening

Mudcrabs Scylla serrata Forskall subjected to zero photoperiod and fed to sitiation for 7 days increased weight by 500%. Growth achieved by cultured crabs under normal photoperiod for 20 days were achieved in 7 days only under photoperiod.

 

CSC

Dr. Minerva I. Morales

 

Use of Yam (Ubi) Vine Cuttings for Tubers Propagation

Tuber production technology for yam that uses tip and middle vine cuttings. The fresh tubers from tip and middle vine cuttings had mean weights of 945.83 grams and 870.83 grams respectively.

 

CSC

Prof. Pablo P. Gualberto

 

Golden Kuhol Crusher Grinder

 

The concept of the Golden Kuhol Crusher-grinder was based from existing hammer mill machines used in crushing and/ or grinding feed materials but comparably low-cost, less laborious, and can easily be operated even by a woman.

 

CSSAC

Engr. Marife L. Pesino

Developed;

For commercialization/ promotion

Development of Integrated Multi-Crop Processing System (Zero Waste Ginger Processing Technology)

 

The project was conceptualized to develop tools and equipment for ginger processing and to provide the integrated mechanized system that will solve the laborious and tedious operations in processing ginger.

 

BU

Engr. Arnulfo P. Malinis

For commercialization/ promotion

 

Smoke Fish Enterprise

 

 

In the study on Physico-Chemical and Microbiological Analysis using Flavor enhancer, it was found out that rice hull is a good source of smoke during fish smoking and bagasse is a good flavor enhancer. PSU endorsed that promotion of the technology of smoked fish because: it is one of the most common preserved fish product marketed in Bicol Region; agro-waste smoking materials such as ricehull and bagasse are available at hand and there is high demand of protein rich food and protein-deficient areas in Bicol Region.

 

PSU

Myrna C. Bigueja

For commercialization

PSU Automated Registrar System ( P@RS )

 

It is an attempt to develop an alternative comprehensive information system that can be implemented to assist the Registrar's Office of Partido State University. It aimed to transform the manual processes on student-related records into a set of on-line computerized services.

 

PSU

Ronnel R. Atole

Nicolas A. Pura

Antonio B. Oquialda

For implementation

Decrowning and Fertilization at Red Bud Stage of Queen Pineapple

 

Combination use of fertilization and decrowning can be recommended at low rate of fertilizer use at flowering. However, depending on the capabilities of farmers and future demand of market, especially foreign market, high rate of fertilization coupled with decrowning may prove useful.

 

DA-V

Inocencio Q. Obrero

 

The Effects of Iodized Salt on the Quality of Bagoong Dilis

 

The study was conducted over a period of six months to one year using iodized salt as the experimental salt and Las Piñas alt (commonly found in the market) as the control. Three runs/ replications were made for each of the two parts salt to seven parts fish. Only one variety of fish was used which is dilid or enchovies. The procedure in mixing bagoong was the recipe recommended by BFAR as founding BFAR brochure.

Sensory evaluation of each run-product was conducted by thirty-five evaluators using seven point scale. There were six products using 1:3 salt to fish ratio and six products using the 2:7 salt and fish ratio.

Results of the sensory evaluation showed (a) for color moderately attractive to very attractive color (b) for odor/ aroma (c) for degree of saltness: moderately to very high degree of saltness and (d) for flavor; like moderately.

Return on investment analysis (ROI) indicated 10-20% profit. Recommendations include using other species of fish and formulation ratio.

 

SSC

Yolanda E. Berdin

Augusto Calabines

 

Pen Culture of Mudcrab (Scylla Serrata Estampador, keenan et.al, 1998) In Mangrove: Analysis of Stocking Density Effects to Growth, Mortality and Production

The pen culture of mud crab in the mangrove areas along Incarizan River within 150 days. Three hundred twelve crablets were purchased and placed in twelve experimental plots which were enclosed in nets supported by bamboo bridge used for feeding and monitoring. The crablets were initially weighed before stocking to determine the amount or percentage of feeds to be given. The crablets were fed twice a day using the broadcast method. Results of the study showed no clear indication that the presence of Mangrove (aveceia sp) prevent cannibalism and enhance the growth or survival.

The result of the experiment after 150 days is very encouraging for fish farmers for two reasons, First, pen culture is very economical in terms of cost both in the construction and the maintenance. Second, it is also ecology friendly because it maintains the normal environment including the mangroves while the process of culturing is going on.

 

SSC

Tito M. Ciruelos

 

 

Eco-Friendly Mangrove Aqulaculture: An Evaluation on the Growth, Survival and Production of Epinephilus coioides, Siganus gutatos and Scatophagus argus

Physico-chemical parameters: Slight variations on water temperature values caused no significant effects in the growth of the subjects. Increase in water temperature was noted during the months of April and May especially during low tides causing the stocks (siganids and spade fish) to surface from the water gasping for oxygen. Salinity values ranged from 10.28 ppt. It was noted that an increase in after temperature correspondingly causes an increase in salinity. This ode of variations occurred from March to June due to occasional rainfall. Wide range of salinity and temperature variations were due to vast water sheds bounding Incarizan river. Higher salinities (26.69 ppt) were observed in the month of April.

Growth and survival: Growth rates of the experimental species over a period of four months observation showed that the monthly growth increment are predicted to be influenced by the capacity of the stocks of feed at the early stage of their lifecycle. It was noted that the grouper (TI) had exhibited better growth performance due to food abundance, T3 and T2 had exhibited inferior growth performance compared to T1 since poor feeding of the test animals was attributed to the limited amount of natural food generated from the natural substrates. At low tides substrates are exposed to sunlight hindering their reproduction when out of the water for number of hours studies conducted on the cultivation of grouper, siganids and spade fish in ponds and cages had exhibited good performance. Culture of the species mentioned in mangrove areas had exhibited no significant difference in terms of growth. Differences in survival and recovery are predicted to be low at the mangrove aquaculture due to the disturbances caused by rats on the structure and occasional dike breakage especially during high tides.

Salinity and temperature variations did not in any way affect the growth performance of the test animals since problems on the Physico-chemical parameters of the water are easily remedied due to incoming tides at high tide.

 

SSC

Ramonito D. Duro

Olga B. Labalan

Dolores C. Huidem

Augusto N. Calabines

 

Pen Culure of Mud Crab (Scylla serrata and S. olivacea Estampador, Keenan, et al, 1998) in Tidal Flat with Existing Mangroves: Verification and Comparative Study

The results of the verification study showed that the wooden gate used in 1,600sq.m.culture of mud crab in tidal flat with existing mangroves is not suitable and applicable, because it does not maintain the required water level inside the pen during low tide. The continuous leakage of water in between the slabs of the wooden gate of the enclosed area partially drains it of water except the ditches or central canals. The study showed also that the draining of water for three consecutive nights is favorable to the rats that prey on the mud crab stocks. Therefore, the water level requirement is a critical factor in preventing rat from preying on crabs.

In the comparative study of two species of crabs, the two-group design was employed as experimental and control group. Results showed that there was no significant difference in survival of the two species of crabs at 0.5 level of probability survival rate of the control group (S. serrata) was noted at 49%, with a better average body weight attainment of 390.322 grams for five months culture period compared to five months 275 g. with the verification study conducted in Puerto Princesa in collaboration with Local Government Units. While the S. olivacea has a survival rate of 87%, it has a lower average body weight of 224.40g. The high mortality rate was observed in S. serrate due to rats preying on stocks when the site was drained of water. Cost-return analysis in one cropping in 800 sq.m. basis showed that the S. serrata species has a return on capital investment (ROI) of 17% compared to –0.37% in S. olivacea. However, it is possible to increase the survival rate and production of S. serrata and its ROI by controlling rat through maintaining the water level inside the pen especially at night time. Likewise, the draining of water from the area should be done early in the morning or mid-afternoon.

 

SSC

Dr. tito M. Ciruelos

Dr. Tarcela F. Detera

Prof. Rafael V. Amene

 

 

Survival and Production of Scylla Serrata Estampador (Keenan et el, 1998) Under Various Shelters in Enclosed Mangroves

The effects of various shelters on survival and yield grams per sq.m. of rearing juvenile Scylla serrata in enclosed mangrove were investigated at Incarizan River , Magallanes, Sorsogon. Mangroves forest was protected during the entire experiment. Four treatments with three replicates were employed. Results of the study showed the optimum yield in grams per sq.m. of Scylla serrata and its profitability was noted on the 4 th month of the rearing periods in the four treatments. The survival and yield in grams per sq.m. of Scylla serrata at the four treatments were significantly different at the 5% level of significance. The three pair comparisons showed that Treatment 2 gave yield t6hat was significantly higher that that of the Treatment 1 (control). The computed mean difference between Treatment 2 and the control exceeds to the computed Least Significant Difference (LSD) test values of 5% level of significance, but not significant in Treatment 3 and Treatment 4. The survival rate was significant in Treatment 4 but not significant in Treatment 2 and Treatment 3. The highest ROI and lowest production cost were observed in the Treatment 2. Cost and Return analysis showed no profit benefit from the 3 rd and 5 th , 6 th and 7 th months of the culture period of all treatments except in Treatment 2 and Treatment 4. After the 5 th month there was significant decline in these two treatments. All treatments became unprofitable beyond this rearing period. Net profit declined and incurred losses dur to mortality as a result of cannibalism. It is also the start of the spawning of female crabs and spawning female crabs do not have market value, they die after egg hatching.

 

SSC

Dr. Tito M. Ciruelos

Dolore C. Huiden

 

 

Comparative Study on Growth of Mud Crabs (Scylla Serrata) Fed on Trash Fishes and Formulated “Kuhol”Diet

Feed Acceptability: On the 1 st week of feed administration to the stock, treatment fed on trash fish responded immediately to the feed while Treatment on formulated diet seems to disregard the feed until such time that they were starve where they start feeding. It was noted that there was a significant difference in the growth of stocks between treatments I and II as revealed during the 1 st sampling period.

Growth and Survival: Based from the results of the different treatments, Treatment I (control) represented a mean growth rate of 1.0734g.per day. Treatment II (fed with formulated diet) 1.1464g per day and Treatment III ( combination of trash fish and formulated diet) 0.09609 g respectively. These values simply show that treatment II had exhibited superior growth rate over Treatments I and III. However, during the 1 st week of feeding, T0II and T-III had shown slight depressions in growth due to the period of feed adjustment. Treatment III had the poorest result implicated by wastage of formulated diet since trash fish was given priority by the stocked animals while formulated diet dis-integrates before these were consumed by the animals.

 

SSC

Ramonito D. Duro

 

Development of New Food Product from Pili Pulp

The researchers determine the appropriate mixture of pili pulp with gabi, oatmeal and cornstarch to produce pudding, fruitcake and crunchy balls. The appropriate proportion of pili pulp tried by the researchers in each of the recipe was: for pudding: ½ c. gabi, and 2c pili pulp; for fruit cake: 1c. oatmeal and 1c pili pulp; for crunchy balls: 1/2c. flour and 2 c pili pulp.

Based on the results, the findings were: The ideal mixture for pudding based from evaluation of students, faculty members and community is treatment 4; for pudding, 1 part mashed boiled gabi and 2 parts pili pulp; for fruit cake, 1 part oatmeal and 1 part pili pulp; for crunchy balls, 1 part cornstarch and 2 parts pili pulp. As to characteristics and level of acceptability, pudding, fruitcake and crunchy balls are moderately attractive in color, moderately pleasant in odor and moderately smooth in texture. Sensory evaluation for pudding, fruitcake and crunchy balls for taste is very much like. In one-week period the ROI of pudding is 39.40%, fruitcake- 47.32 % and crunchy balls- 33.33%.

For the pulp nutritional analysis findings are: protein 8%, 33.6 % carbohydrates 45.8% and ash 9.2%.

 

SSC

Prof. Sonia V. Catabian

Dr. Tarcela F. Detera

 

Prawn-Gracilaria Polyculture in Net Pen Enclosure Within Mangrove Areas

The results of the study on ‘Prawn-Gracilaria Polyculture conducted within Net Pen Enclosure in Mangrove had manifested a strong indication that aquaculture activities in mangrove can co-exist with mangrove forest and increase production up to 1,562.44 kg/ha. This production is comparable and proven higher (4.03%) than the production per hectare in the conventional extensive bangus culture under pond condition (1,500kg).

In terms of growth rate of prawn results showed a 31% superiority (ave body wt.- 14.45) over the results achieves on the experiment conducted by SEAFDEC/AQD at Alacagan, Banate, iloilo (ABW=6.98g in 4 mo.) on shrimp pond using mangrove pond as water filter. This study showed that the lesser the stocking density (T-I) increase survival rates ad weight gain (62%= 18.431 g) of prawn compared to T-II (12.45112g) and T-III (11.7969g) respectively.

Results of this experiment also revealed a faster growth rates of prawn during the second and third month of their life cycle when prawn juveniles had achieved an average body weight of 3-5 grams. Generally, high stocking density in Prawn-Gracilaria Polyculture had considerable affected, survival growth of prawn (ave. 44.6% and 14.45 g. respectively) due to insufficiency on food, dissolve oxygen and space requirement. Predation and inconsistent salinity and temperature ranges had aggravated low survival and production.

Seaweeds growth did not show an admirable result (126g) due to grazing of prawn on generating spores and new growths. The growth of mangroves was never affected during the culture period due to occasional roots exposures on lowest-low tides. Mangrove Aquaculture, therefore can co-exist with mangrove forest, contributes to the mangrove rehabilitation and resource management while generating an income as advocated by Trono (1987).

 

SSC

Ramonito D. Duro

Yolanda E. Berdin

 

Shrimp Gracilaria Polyculture in Net Pen Enclosure Within Mangrove Area: An Evaluation

The experimental unit used in study 1 (propagation of Gracilaria) was utilized in this study. Results in this study followed the trend, however, at a very minimum degree. Gracilaria cultivated with prawn gave a little influence to the growth rate of subjects (Mean: 15,357 g) as compared to the results of the subjects were greatly affected by unfavorable water parameters especially during summer season. The growth depressions manifested particularly in treatment 2 and treatment 3 were attributed to high stocking densities, insufficient supply of dissolved oxygen and the salinity and temperature values. The stunted growth of seaweeds was caused mainly in siltation, the grazing of vegetarian entrants species such as rabbit fishes and mullets aside from the prawn consumption of their survival.

 

SSC

Ramonito D. Duro

Yolanda E. Berdin

 

Coconut Embryo Culture Technology

•  Y3 (with or without AC) and MS nutrients formulations (with AC) are comparative and are better than White's medium for in vitro culture of coconut embryos

•  Technique for embryo culture of coconut developed

•  The technique is being used to mass produce embryo cultured makapuno seedlings

•  technique for routine collection of large numbers of embryos for culture developed

•  further improvement of the technique for embryo rescue of Makapuno which allow higher success rate in ex vitro transplanting

•  Germination of embryos was shortened from 6 weeks using White's medium to three weeks in Eeuween's medium with or without activated charcoal or in Murashige and Skoog's with AC

•  Initial findings showed that incorporation of 2 ppm NAA or IBA on the last subculture initiated more and longer roots. But with improved protocol, NAA (10-20ppm) may be incorporated only in the culture medium only for the hart-to-root cultures

•  The length of time to transplant in vitro grown seedlings was also reduced from 6.8 to 4.6 months

•  11 to 12 month old nuts are the appropriate sources of embryos for coconut embryo culture

•  Germination of coconut embryos in Y3 liquid medium was not affected by neither water source and pH adjustment. Further incubation of seedlings showed that pH adjustment is a critical factor. The culture of coconut embryos could use either tap or glass distilled water and either with or without agar

•  Regardless of the cultivars, higher germination rate, enhanced vegetative growth and development, and profuse root formation of embryos were consistently noted following the ARC's that the Assy-Bah's protocol. Results indicate that the ARC embryo culture procedure is optimum for germination, growth and development of coconut zygotic embryos in vitro.

•  Any brand of AC can be incorporated in the medium for the in vitro culture of coconut embryos.

•  An upgraded hybrid embryo culture protocol is currently being used to culture coconut embryos. Results showed improvement in terms of earlier leaf and root formation, higher percentage of cultures with simultaneous shoot and root formation, higher percentage of transplantable seedlings with 5-7 months and higher recovery rate and ex vitro survival.

•  Makapuno seedlings are being propagated using hybrid embryo culture protocol.

•  The upgraded hybrid embryo culture protocol is being used to mass produce other soft endosperm coconut mutants like Lono and Langadngad.

Tissue Culture Division

PCA-ARC

 

Technology Generated

The Use of Botanical Biocides for the Ocntrol of Major Pests of Coconut and Intercrops

•  Appropriate bioassay procedures suitable for laboratory and small scale field screening of promising pesticidal plants were devised and tested for the first time against major coconut pests and various intercrop pests.

•  Under laboratory conditions, aqueous crude of pesticidal plants were found against the following pests:

Pests of coconut

•  Phinoceros beetle Oryctes rhinoceros

Aqueous extract of fresh Derris elliptica (tubi) roots was very effective against rhinoceros beetle larvae. Using concentration of 1.3 (wt/vol) and higher and incorporated into the reari8ng medium, larvae died within 1-3 days after treatment. Aqueous extracts of other plant species like leaves of Azadirachte indica (neem), Vitex negundo ( Lagundi) and Gliricidia sepium ( kakawate) also resulted in larval mortalities one week after treatment.

 

•  Leafminer, Promecotheca sp.

Aqueous extracts of fresh tubi roots and dried seeds of either Annona muricate ( guyabano), neem and Croton tiglium (tuba-tuba) sprayed on infested coconut leaves killed adults of the coconut leafminer 3 to 6 days after treatment. Highest mortalities were obtaines at 1:3 (wt/vol) concentrations.

 

•  Coconut Meal bug

Aqueous extracts at 1:1 (wt/vol) concentration of fresh tubli roots, neem seeds and tuba-tuba fruits were found highly effective against all stages of the mealybug. Lower mortality rates were obtained with aqueous extracts of leaves of Jatropha curcas (tubing bakod) , Coleus amboinicus (oregano) , Tagetes erecta (marigold yellow) , lagundi, tuba-tuba , curcuma (luyang dilaw) and Chrysanthemun sp. (chrysanthemum). Mealybug nymphs were more susceptible than adults to botanical biocides.

 

•  Palm weevil, Rhynchoporus sp.

Extracts of fresh tubli roots incorporated into rearing media of palm weevil larvae resulted in 100% mortality. Larvae of all stages were killed 3 days after treatment.

 

•  Scale insects

Spraying aqueous extracts of fresh or dried tubli and Tinosphora rumphii (Makabuhay) were effective in controlling scale insects. Aqueous extracts at 1:1 (wt/vol) concentration resulted in 100^ mortality five days after treatment.

 

Pests of Intercrops

Spraying thrips-infested Piper betel (buyo) leaves with aqueous extracts of fresh tuba-tuba fruits or dried tuba-tuba seeds or neem seeds at 1:1 and 1:2 (wt/vol) concentration resulted with high mortality in both adults and nymphs 1-3 days after treatment.

 

Nymphs and adults of blackpepper flatids were susceptible to aqueous extracts of fresh tubli roots 1:2 (wt/vol) concentration. Up to 92% mortality was observed 1-3 days after spraying infested black pepper leaves.

 

A 1:2 (wt/vol) concentration of aqueous extracts of fresh or dried tubli roots, dried tuba-tuba seeds and hot pepper fruits incorporated into the rearing media resulted in Ostrinia furnacalis ( cornborer) larvae mortalities 1-2 days after treatment.

 

 

 

Entomology Division

PCA-ARC

 

Technology Generated

DNA Marker Technologies for coconut genetic analysis and genome mapping

•  Different PCR-based DNA marker types )randomly amplified polymorphic DNA-RAPD, inverse sequence-tagged repeats-ISTR, amplified fragment length polymorphism-AFLP and microsatellite-MS) were used to construct linkage maps in coconut( cocos nucifera L., 2n=32). A total of 2033 markers have been mapped so far. Linkage maps for 6 genotypes were established on the basis of four coconut mapping populations: East African Tall (EAT0707) x Pemba Red Dwarf (PRD), EAT1011 x Rennel Island Tall (RIT0710), Malayan Yellow Dwarf (MYD20) x Laguna Tall (LAGT07) and Cameroon Red Dwarf (CRD) x RIT. The current status of the 6 individual maps is:

 

MYD20, 216 markers on 16 linkage groups, map length of 1459 centimorgan (cM)

LAGT01, 293 markers on 16 linkage groups, map length of 2533 cM

EAT0707, 707 markers on 16 linkage groups, map length of 2083 cM

EAT1011, 338 markers on 16 linkage groups, map length of 2360 cM

RLT0710, 204 markers on 16 linkage groups, map length of 1492 cM

RIT, 275 markers on 16 linkage groups, map length of 1860 cM

 

In visualizing the DNA fingerprints resulting from these markers, silver nitrate staining procedure is being used in place of radioactive 32P. Silver staining is less expensizve, not hazardous to handle and environmental-friendly procedure.

 

Inverse sequence tagged repeats (ISTR) developed primarily for coconut was also applied and used to characterize a fungal pathogen, Phytophthora palmirona, causing coconut bud and nuts rots.

 

•  QTL analysis for the trait early germination identified six loci on five different linkage groups of MYD and LAGT. These QTLs correlate with early flowering and yield, representing important traits in coconut breeding.

•  Eight MADS-box, 2 MYB and 3 homeobox genes (belonging to the class 1 Knox genes) have been sequenced and (in part) mapped. These genes serve as candidate genes in coconut that i), represent resistance loci (RGL) tro biotic stress and ii) control important processes in plant development and differentiation such as leaf development and flower organogenesis.

Virology- Biochemistry Division

PCA-ARC

 

Technology Generated

Diagnostic assay for Cadang-cadang disease of coconut

•  Large number of samples (2000 samples using dot-blot and 50-60 samples using northern blot) can be processed

•  It has a detection –end point of 3.6 ppb for northern-blot MHA and 0.36 ppb for dot blot MHA. Early detection of the disease is made possible

•  With this very sensitive detection assay, some physical and chemical properties of the causal agent of the disease were established. The following were some of the recent findings:

•  Crude and purified CCVd preparations are degraded after autoclaving at 117 ° C for 15-30 min and are not degraded after pasteurization at 68-83 ° C at varying length of time and microwave exposure for 1 to 3 min.

•  CCCVd tolerate freezing and thawing

•  CCCVd is sensitive to pancreatic Rnase and Rnase Ty but unsensitive to phenol treatment

•  Alcoholic solution degrades CCCVd and is used in decontaminating laboratoty wares but highly resistant to UV and ionizing radiation

 

•  Validation of previous findings that Laguna, Batangas andCavite are cadang cadang disease-free areas

•  Colorometric detection method is being used in place of radiolabeled probe. This is less expensive, less health hazard to the handlers and environmentally-friendly.

Virology- Biochemistry Division

PCA-ARC

 

Technology Developed

 

 
 

 

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NOVEMBER 2005

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