FIBER INDUSTRY DEVELOPMENT AUTHORITY
BU Compound, Legazpi City
Accomplishment of the Fiber Industry Development Authority, Region V on Research and Development for the Year 2004
PROFILE OF FIDA BCARRD-RMIS REPRESENTATIVE
Name: EMMA O. OLOTEO
Agency: Fiber Industry Development Authority
Position: Senior Agriculturist
Field of specialization: Agriculture major in Agronomy
College BU College of Agriculture , Guinobatan, Albay
(Bachelor of Science in Agriculture)
Masteral BU Graduate School , Legazpi City
( Master of Science in Agronomy)
Doctoral Camarines Sur, State Agricultural College
(Ph. D. in Plant Science major in Agronomy)
Articles/ Researches/ Books Published:
Australian Plant Pathology (2000) 29:69
DISEASE NOTES OR NEW RECORDS
First record of natural infection of abaca ( Musa textiles) with banana bract mosaic potyvirus in the Philippines
M. Sharman A , C.F. Gambley A , E.O. Oloteo B , R.V.J. Abgona C and J.E. Thomas A
A Department of Primary Industries, Queensland Horticulture Institute, 80 Meters Road , Indooroopilly,
Queensland 4068 Australia
B Fiber Industry Development Authority, Region 5, BU Compound, Legazpi City , Philippines
C Fiber Industry Development Authority, Asiatrust Bank Annex Building, 1424 Quezon Avenue , Quezon City , Philippines
Corresponding author J.E. Thomas (Email firstname.lastname@example.org )
The first record of natural infection of abaca ( Musa textiles ) with banana bract mosaic potyvirus (BBrMV) is reported from the Philippines . Symptoms sometimes included the chlorotic spindle-shaped lesions typical of BBrMV on banana.
Abaca (Manila hemp, Musa textiles Nee) is closely related to edible bananas and plantains ( Musa spp.). M. textiles is native to the Philippines , and this country supplies about 85% of total world demand for this natural fibre crop. Abaca bunchy top and abaca mosaic (AMD) diseases are major constraints to production, and an eradication program has been implemented for their control. AMD is caused by abaca mosaic potyvirus (AbaMV0, which is closely related to sugarcane mosaic potyvirus (SCMV; Thomas et al. 1997).
Samples of abaca with suspected symptoms of AMD were collected from Tabaco, Albay Province , Philippines . Samples were M2 (cv. Luno), M3 (cv. Musa tex 51) and M4 (cv. Musa tex 50) from location 1 and M1 (cv. Musa tex 52), M5 (Musa tex 51) and M6 (cv. Musa tex 50) from location 2.
Samples M2, M3 and M4 gave positive ELISA reactions with SCMV antibodies with A 410nm values of 0.77-1.94. Healthy abaca and samples M1, M5 and M6 had values of 0.00 in the same tests (Bioreba ELISA kit, cat. No. 140412/ 140422. By contrast, samples M1, M5 and M6 were positive by ELISA for banana bract mosaic potyvirus (BBrMV) with A 410 nm values in the range of 0.70-1.05 in double and triple antibody sandwich ELISA (Thomas et al . 1997); Thomas, unpublished), compared to values of 0.01-0.05 for samples M2, M3, M4 and healthy abaca in the same tests. These results were confirmed by immunocapture PCR with AbaMV – and BBrMV – specific probes for microplate detection of amplicons (Sharman, Dietzgen and Thomas, unpublished). The presence of BBrMV in sample M6 was also confirmed by immunosorbent electron microscopy and decoration with antibodies to BBrMV.
Samples M2, M3 and M4 had typical symptoms of AMD (Diekmann and Putter 1996). M1 showed raised leaf veins and broad chlorotic stripes similar to tissue culture off-types, whereas M5 was degraded and symptoms were uncertain. Sample M6 showed leaf mosaic symptoms, including chlorotic spindle-shaped lesions typical of BBrMV in banana.
This is the first report of BBrMV naturally infecting abaca.
Thomas, J.E., Geering, A.D.W., Gambley, C.F., Kessling, A.F. and White, M. (1997) – Purification, Properties and diagnosis of banana bract mosaic potyvirus and its distinction from abaca mosaic potyvirus. Phytopathology 87: 698-705.
Diekman, M. and C.A.J. Putter (1996) – FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm. No. 15. Musa 2 nd edition. Food and Agriculture Organisation of the United Nations, Rome/International Plant Genetic Resources Institute, Rome .
ccepted 24 January 2000.
Hereunder are the accomplishment on Research and Development of FIDA V from January to December 2004:
Research and Development Projects
A total of six (6) studies/projects were implemented in the Bicol Region under different fund sources. These are listed below.
FIDA Funded Project/Study
Collection, Evaluation and Characterization of Abaca Varieties, Hybrids and Strains. E.O. Lomerio, Oloteo, E.O., Infante, E.B., Padua , F.
Progress of the study : Only three (3) varieties were collected from Poblacion, Rapu-rapu, Albay. To date, collection of abaca varieties planted and maintained at Sorsogon Fiber Seedbank in San Juan , Casiguran, Sorsogon totaled to 111. Care and maintenance of such varieties was being undertaken by following the FIDA's Abaca Package of Technology.
CFC-UNIDO Assisted Projects
National Yield Trial on Abaca. E. B. Infante .
Salient Findings : Preliminary results on the different yield parameters revealed that the top three abaca varieties were Inosa, Musa tex 51 and Linawaan.
National Screening of Abaca Varieties for Resistance to Bunchy-top Disease. E.O. Oloteo, Navarro, I.M., Siapno, J.O.
Salient Findings : Results showed that the highest in the degree of resistance to bunchy-top was Inosa followed by Maguindanao, Abuab, Linawaan, Musa tex 51, Tangongon, Lausigon and the lowest was Laguis.
National Screening of Abaca Varieties for Resistance to Mosaic Disease. E.O. Oloteo, Navarro, I.M., Siapno, J.O.
Salient Findings : For resistance to mosaic, the ranking of the varieties was as follows: Linawaan, Abuab, Musa tex 51, Maguindanao, Laguis, Lausigon, Tangongon and Inosa.
DA-BAR Funded under the GMA-HVCC Program
Indexing of Micropropagated Abaca in Bicol
The project was implemented by Albay Abaca Diagnostic Laboratory, three (3) Tissue Culture Laboratories located in Albay, Catanduanes and Sorsogon, and Sorsogon Fiber Seedbank in San Juan , Casiguran, Sorsogon.
The project involved the use of biotechnology in plant propagation and virus detection.
The objective was to produce certified virus-free abaca planting materials through tissue culture technique.
Virus detection was done through Enzyme-linked Immunosorbent Assay or ELISA which is a serological method of virus detection and identification.
A total of 56 leaf samples were collected and indexed of which 39 were identified as virus-free. The virus-free abaca suckers were used as sources of explant for the establishment of initial cultures in-vitro . The number of virus-free tissue culture plantlets that were produced and acclimatized reached 57,601 representing 130.70% accomplishment out of the 44,070 target. The plantlets that were distributed to LGU nurseries totaled to 37,043.
Under this project one “Protected Nursery” was constructed in Sorsogon Fiber Seedbank in San Juan , Casiguran, Sorsogon. Virus-free abaca were maintained in this nursery as sources of virus-free explants to be used in establishing initial cultures at the Abaca Tissue Culture Laboratories.
The research component of the project was “In-vitro Elimination of Bract Mosaic Virus through Chemotherapy”. Revitol was the chemical used for virus elimination. The protocol on the inoculation of AbaBrMV was aseptically formulated. Meristem culture was undertaken.
Abaca Disease Management in the Bicol Region
The task of the Albay Abaca Diagnostic Laboratory in the Bicol Region was to identify virus-free abaca. With the use of ELISA, a total of 300 abaca suckers were identified as virus-free. These virus-free suckers were used by the 3 Tissue Culture Laboratories in Albay, Sorsogon and Catanduanes in establishing initial cultures.
The total number of tissue-cultured plantlets produced was 22,810 with 24 recipients. These plantlets were used to replant abaca areas formerly rogued due to viral infection.
Tissue Analysis of Bract Mosaic Infected Abaca
Emma O. Oloteo
The study was conducted in June 2004 with the objective of determining the nutrient composition of bract mosaic disease infected abaca. Leaf samples of infected abaca with mild, moderate and severe symptoms were collected including symptomless. The samples were analyzed at the Department of Agriculture, Bureau of Soils, Naga City .
Results of the study showed that the macronutrients, nitrogen, phosphorous, potassium, calcium and magnesium are not the limiting factors in the expression of bract mosaic symptoms; the same with the micronutrient copper. However, the micro nutrient iron was very much higher in the symptomless samples having 1400 ppm as compared to the samples with mild, moderate and severe symptoms having 540 ppm, 600 ppm and 340 ppm respectively.
Participatory Action Research (PAR)
This activity aimed to enhance the skills of farmer-leaders with the end-view of threshing out solutions to their technical problems in abaca production through the conduct of farm experiments and researches as a means to complement the formal research undertakings by the Authority. The PARs conducted were as follows:
Effect of Farming System Adopting the Package of Technology Recommended by the Authority in Abaca Production
Farmer-researcher: Mr. Michael Puentebella
Location: Ogob, Malinao, Albay
Adoption of Improved Herbicide Dispenser in Eradication of Virus Infected Abaca Plants
Farmer-researcher: Mr. Florentino Cedano
Location: Lipac, Catagbacan, Goa, Cam . Sur
Differentiation of Farmers Income in Producing Excellent Cleaning of Fiber over Low Grades Using Mobile Spindle Stripping Machine (MSSM) and Handstripping Method
Farmer-researcher: Mr. Rafael Estorninos
Location: Bentuco, Gubat, Sorsogon
Farmers Profitability in Selling Abaca Tuxies versus Dry Fiber Traditionally Handstripped Fiber
Farmer-researcher: Mr. Dario Dayawon
Location: Paniquihan, Baras, Catanduanes
Distribution of Information Materials
There were a total of 137 various information materials distributed to 91 recipients. The information materials distributed include Technoguide on Abaca, Salago, Pineapple, Maguey and Kapok; Trade Inquiries/Opportunities; The Philippine Abaca Industry, Fiber Market Situation, brochures and posters on Abaca Bunchy-top and Mosaic.
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